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low-glucose minimum essential medium (mem) containing neaa  (Procell Inc)

 
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    Procell Inc low-glucose minimum essential medium (mem) containing neaa
    Low Glucose Minimum Essential Medium (Mem) Containing Neaa, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low-glucose+minimum+essential+medium+(mem)/minimum+essential+medium+non+essential+amino+acids++mem+neaa/pmc11414528-172-12-16
    Average 90 stars, based on 1 article reviews
    low-glucose minimum essential medium (mem) containing neaa - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Enhancing diabetic foot ulcer healing: Impact of the regulation of the FUS and ILF2 RNA‑binding proteins through negative pressure wound therapy.
    Article Snippet: The HaCaT cells were cultured in low‐glucose Minimum Essential Medium (MEM) containing NEAA (cat. no. MP150410; Procell Life Science & Technology Co., Ltd.) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and cultured at 37 ̊C in a cell culture incubator maintained at 5% CO2.



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    Image Search Results


    ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively in PBS (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture medium or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.

    Journal: PLOS Biology

    Article Title: The C-terminus of the prototypical M2 muscarinic receptor localizes to the mitochondria and regulates cell respiration under stress conditions

    doi: 10.1371/journal.pbio.3002582

    Figure Lengend Snippet: ( A ) 96-well plate reader measurement of the increase of EGFP fluorescence relative to Sirius in COS-7 cells transfected with the bicistronic Sirius-M 2 i3(417n)-EGFP plasmid and starved for 3 h respectively in PBS (left panel) ( n = 3 transfections, 16 wells) and HBSS (right panel) ( n = 1 transfection, 16 wells). Significance values reported in the graphs were determined by a one-tailed Student t test, p -values: **** p < 0.0001; ** 0.001< p <0.01. ( B ) Single-cell measurement of EGFP vs. SIRIUS expression in HEK293 cells, in basal conditions and upon starvation. Lines are linear fits (note log-log scale). ( C ) HEK293 cells were sequentially transfected with pMito-GFP1-10 (24 h) and M 2 -GFP11 (8 h, of which 6 h under starvation conditions) and then grown in full culture medium or ( D ) starved for 6 h after transfection of M 2 -GFP11. Shown are EGFP fluorescence (left, with DIC inset), Mitotracker (center), and overlay (right). Scale bars are 10 μm. Source data for panels A and B, and raw images related to panel C and D can be found in . IRES, internal ribosome entry site; PBS, phosphate-buffered saline.

    Article Snippet: After washing each well with 2 ml PBS, the old medium was exchanged with 1.5 ml low glucose Minimum Essential Medium (MEM) (Gibco, Thermo Scientific) supplemented with 1% P/S, in order to create a stress-like cell starvation condition.

    Techniques: Fluorescence, Transfection, Plasmid Preparation, One-tailed Test, Expressing, Saline